expression of h11 antigen (habp) in benign and malignant cervical tumor tissue using mab h11b2c2

background: the development and progression of human tumors is accompanied by various cellular biochemical and genetic alterations. these events include tumor cells interaction with extracellular matrix molecules including hyaluronan and hyaluronan binding protein (ha-habp). hyaluronan is a large polysaccharide associated with pericellular matrix of proliferating, migrating cells, regulation of hyaluronan expression during cervical ripening.  its implication in malignant transformation, tumor progression and with the degree of differentiation in various invasive tumors has well accepted. it has been well known the role of ha receptors in tumor growth and metastasis in various cancer tissues. methods: the procedure carried out by using 10 benign and 30 different grades of cervical cancer tissue samples to see the expression of habps protein by western blotting method. results: this study is based on the clinical work of benign tissue and cervical cancer tissue samples. the results indicated that it is a habp. it conforms that 57kd protein could be a common marker. conclusion: the habp of 55-57kd and 30kd proteins, which were observed in western blot. it was shown by using a specific probe bha for the detection of habp, which recognizes all types of hyaladherins.  data suggests over-expression of h11 antigen (habp) in tumor cells and it is an important parameter and a clinical diagnostic marker for all progressive human tumors. key words: hyaluronan (ha); h11 antigen; hyaluronan-binding proteins (habp); cervical cancer; bha (biotinylated hyaluronic acid) references 1. toole b.p. hyaluronan and its binding proteins, the hyaladherins. curr. opin. cell biol.1990; 2:839–44. 2. fenderson b.a, stamenkoyic l, aruffo a. localization of hyaluronan in mouse embryos during implantation, gastrulation and organogenesis. differentiation. 1993;54: 85-98. 3. gakunga p, frost g, shuster s, cunha g, formby b, stern r: hyaluronan is a prerequisite for ductal branching morphogenesis. j. development 1997, 124,3987-3997. 4. turley e.a. hyaluronan and cell locomotion.cancer metastasis rev. 1992; 11: 21-30. 5. degrendele hc, estess p, picker lj, siegelman mh. cd44 and its ligand hyaluronate mediate rolling under physiologic flow: a novel lymphocyte-endothelial cell primary adhesion pathway.j exp med. 1996; 183:1119-30. 6. knudson c.b, knudson w. hyaluronan binding proteins in development, tissue homeostasis and disease. faseb j. 1993; 7- 1233-41. 7. underhill c. cd44, the hyaluronan receptor. j.cell sci. 1992;103:293-8 8. turley e. a, noble pw, bourguignon l. y. signaling properties of hyaluronan receptors. j. biol. chem. 2002; 277: 4589–92. 9. ponta h, sherman l, herrlich p. cd44: from adhesion molecules to signaling regulators. nature rev mol. cell biol, 2003; 4: 33–45. 10. bourguignon l.y. cd44-mediated oncogenic signaling and cytoskeleton activation during mammary tumor progression.j. mammary gland biol neoplasia. 2001; 6, 287–97. 11. thorne r. f, legg j. w, isacke c. m. the role of the cd44 transmembrane and cytoplasmic domains in coordinating adhesive and signalling events.j. cell sci, 2004; 117:373–80. 12. banerjee s.d, toole b.p .the determinants of migrating with a pre-arranged job and of the initial duration of urban unemployment: an analysis based on indian data on rural-to-urban migrants.j dev econ. 1991; 36:337-51. 13. laemmli uk. cleavage of structural proteins during the assembly of the head of bacteriophaget4.nature. 1970; 227:680-5. 14. boregowda  rajeev k, hitesh n appaiah, manjunath siddaiah, sunil b kumarswamy, sunila sunila, thimmaiah kn, et al. expression of hyaluronan in human tumor progression.j carcinog.2006;5: 2, 1477-85. 15. grammatikakis n, grammatikakis a, yoneda m, yu q, banerjee sd ,  toole bp. a novel glycosaminoglycan-binding protein is the vertebrate homologue of the cell cycle control protein cdc37. j biol chem .1995; 270:16198-205. 16. huang l, grammatikakis n, yoneda m, banerjee sd , toole bp.molecular characterization of a novel intracellular hyaluronan- binding protein.j biol chem 2000; 275: 29829-39.